You didn't really give enough info to diagnose the problem, but OK, let's try some things. First, I noticed you have VFO B selected. Try selecting VFO A.
Secondly, in the main window, you have a button labeled OPT. Click that to get a drop down menu. Under that menu is a selection to calibrate the I/O differences. I don't have SDRUno in front of me right now, so I don't remember the way it's labeled, but try to find that and make sure it's set to auto.
Finally, click SETT in the upper left corner of the main window and find the CAL tab. click that and go to one of the WWV frequencies where you have a good signal. It could be 5.000, 10.000, 15.000, or 20.000 MHZ. Set up on the SAM mode the way the instructions tell you in the CAL box, and click the button. That will calibrate your SDR.
Also, look at this thread: viewtopic.php?f=10&t=1914
Hopefully one of these steps will fix the issue. If not then do a reset of the software and start over.